Extracting DNA
from fruits

🍌🍓🥝

Materials


Dish liquid soap 10 ml

Water 110 ml

Alcohol 20 ml

Half teaspoon of salt

Stainer

Tweezers  

3 measurement cups

Ziploc bags 

Ice

Banana*

Strawberry*

Kiwi*



Reagents and samples


Banana*

Strawberry*

Kiwi*

Dish soap 

Water

Alcohol 

Salt


Procedure


Prepare the lysis solution: Mix the water, the dish soap and the salt. I prepared the same amount of solution to use on kiwi, strawberry, and banana.




Mash the fruit in a Ziploc bag with the lysis solution. Mash very well to release the greatest amount of DNA from the cells.




Filter the mix, this liquid contains the DNA dissolved in it.


Precipitate the DNA! With alcohol. Add alcohol to the mixture and wait for 5 minutes. The alcohol has to be cold; we can put it in the fridge for fifteen minutes or either keep it on ice during the procedure.




And finally here it is! That white slime forming in the middle of the alcohol layers is DNA.







More DNA can be seen from the banana mixture than from the kiwi and strawberry.



Just for fun; I tried to obtain some DNA.

From strawberry

From banana 

    It is fascinating how we can learn from home by performing simple procedures with equipment and ingredients that we use every day in our kitchens.



 





Comments

  1. EricaMarch 30, 2022 at 5:35 PM

    Seriously loving this. A bit more on the theory and this blog would be 100%.

    ReplyDelete

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